2017-04-24 15:34:122025-05-12 20:45:13
description
unknown
similar to magnesium exporter
locus
BSU09590
BSU_09590
geneLength
1383
1386
ec
outlinks
bsu
BSU09590
BSU_09590
The protein
Protein family
UPF0053 family (according to Swiss-Prot)
[SW|UPF0053 family](according to UniProt)
The protein
Paralogous protein(s)
[[protein|MpfA]], [[protein|YrkA]], [[protein|YqhB]], [[protein|YugS]]
[[this]]
The protein
[SW|Domains]
[SW|DUF21 domain] (7-202)
2 [SW|CBS domain]s (221-280, 290-347)
[SW|CNNM transmembrane domain] (aa 1-202) (according to UniProt)
[SW|DUF21 domain] (7-202)
2 [SW|CBS domain]s (aa 221-280, aa 290-347) (according to UniProt)
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane (according to UniProt)
Biological materials
Mutant
MGNA-A698 (yhdT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/698 NBRP B. subtilis, Japan]
MGNA-A698 (yhdT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/698 NBRP B. subtilis, Japan]
BKE09590 (Δ[[gene|yhdT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAACCCTATCCTCTCA, downstream forward: _UP4_AGCGAGAAATAAGCTCAAGG
BKK09590 (Δ[[gene|yhdT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAACCCTATCCTCTCA, downstream forward: _UP4_AGCGAGAAATAAGCTCAAGG
Biological materials
Expression vector
pGP2928 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
References
The protein
Structure
[PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)
Biological materials
Expression vectors
pGP2928 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab